| FNDC5

Fibronectin type III domain-containing protein 5, the precursor of irisin, is a type I transmembrane glycoprotein that is encoded by the FNDC5 gene.Irisin is a cleaved version of FNDC5, named after the Greek messenger goddess Iris.Fibronectin domain-containing protein 5 is a membrane protein comprising a short cytoplasmic domain, a transmembrane segment, and an ectodomain consisting of a ~100 kDa fibronectin type III (FNIII) domain.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	500	250	125	62.5	31.25	15.62	7.81	0
OD	2.67	2.05	1.48	0.97	0.67	0.42	0.27	0.09
Corrected OD	2.58	1.96	1.39	0.88	0.58	0.33	0.18	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human FNDC5 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	95
Plasma(n=8)	91-105	102
Tissue Homogenates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Human FNDC5 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Tissue Homogenates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	88-108	105-115
	Average (%)	94	98	108